Use of new endophytic fungi as pretreatment to enhance enzymatic saccharification of Eucalyptus globulus.
Identifieur interne : 000775 ( Main/Exploration ); précédent : 000774; suivant : 000776Use of new endophytic fungi as pretreatment to enhance enzymatic saccharification of Eucalyptus globulus.
Auteurs : Raquel Martín-Sampedro [Espagne] ; Úrsula Fillat [Espagne] ; David Ibarra [Espagne] ; María E. Eugenio [Espagne]Source :
- Bioresource technology [ 1873-2976 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- microbiologie : Bois, Eucalyptus.
- métabolisme : Bois, Eucalyptus, Laccase.
- méthodes : Biotechnologie.
- physiologie : Ascomycota, Endophytes, Trametes.
- Espagne, Hydrolyse, Métabolisme glucidique.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Laccase.
- metabolism : Eucalyptus, Wood.
- methods : Biotechnology.
- microbiology : Eucalyptus, Wood.
- physiology : Ascomycota, Endophytes, Trametes.
- Carbohydrate Metabolism, Hydrolysis, Spain.
Abstract
New endophytic fungi are assessed for the first time as pretreatment to enhance saccharification of Eucalyptus globulus wood. The fungi are all laccase-producing ascomycetes and were isolated from eucalyptus trees in Spain. After five endophytes had been assayed alone or in combination with white-rot fungus Trametes sp. I-62, three were pre-selected. To improve sugar production, an autohydrolysis pretreatment was performed before or after fungal treatment. Pretreatment increased sugar production 2.7 times compared to non-pretreated wood. When fungal and autohydrolysis pretreatments were combined, a synergistic increase in saccharification was observed in all cases. Endophytic fungi Ulocladium sp. and Hormonema sp. produced greater enhancements in saccharification than Trametes sp. I-62 (increase in sugar yields of 8.5, 8.0 and 6.0 times, respectively), demonstrating the high potential of these new endophytic fungi for saccharification enhancement.
DOI: 10.1016/j.biortech.2015.07.088
PubMed: 26255602
Affiliations:
Links toward previous steps (curation, corpus...)
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Eugenio</name><affiliation wicri:level="2"><nlm:affiliation>INIA - CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, Ctra. de La Coruña km 7.5, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>INIA - CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, Ctra. de La Coruña km 7.5, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author></analytic><series><title level="j">Bioresource technology</title><idno type="eISSN">1873-2976</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota (physiology)</term><term>Biotechnology (methods)</term><term>Carbohydrate Metabolism (MeSH)</term><term>Endophytes (physiology)</term><term>Eucalyptus (metabolism)</term><term>Eucalyptus (microbiology)</term><term>Hydrolysis (MeSH)</term><term>Laccase (metabolism)</term><term>Spain (MeSH)</term><term>Trametes (physiology)</term><term>Wood (metabolism)</term><term>Wood (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Ascomycota (physiologie)</term><term>Biotechnologie (méthodes)</term><term>Bois (microbiologie)</term><term>Bois (métabolisme)</term><term>Endophytes (physiologie)</term><term>Espagne (MeSH)</term><term>Eucalyptus (microbiologie)</term><term>Eucalyptus (métabolisme)</term><term>Hydrolyse (MeSH)</term><term>Laccase (métabolisme)</term><term>Métabolisme glucidique (MeSH)</term><term>Trametes (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Laccase</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Eucalyptus</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biotechnology</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Bois</term><term>Eucalyptus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Eucalyptus</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Bois</term><term>Eucalyptus</term><term>Laccase</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Biotechnologie</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Ascomycota</term><term>Endophytes</term><term>Trametes</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Ascomycota</term><term>Endophytes</term><term>Trametes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Carbohydrate Metabolism</term><term>Hydrolysis</term><term>Spain</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Espagne</term><term>Hydrolyse</term><term>Métabolisme glucidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">New endophytic fungi are assessed for the first time as pretreatment to enhance saccharification of Eucalyptus globulus wood. The fungi are all laccase-producing ascomycetes and were isolated from eucalyptus trees in Spain. After five endophytes had been assayed alone or in combination with white-rot fungus Trametes sp. I-62, three were pre-selected. To improve sugar production, an autohydrolysis pretreatment was performed before or after fungal treatment. Pretreatment increased sugar production 2.7 times compared to non-pretreated wood. When fungal and autohydrolysis pretreatments were combined, a synergistic increase in saccharification was observed in all cases. Endophytic fungi Ulocladium sp. and Hormonema sp. produced greater enhancements in saccharification than Trametes sp. I-62 (increase in sugar yields of 8.5, 8.0 and 6.0 times, respectively), demonstrating the high potential of these new endophytic fungi for saccharification enhancement.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26255602</PMID><DateCompleted><Year>2016</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2976</ISSN><JournalIssue CitedMedium="Internet"><Volume>196</Volume><PubDate><Year>2015</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Bioresource technology</Title><ISOAbbreviation>Bioresour Technol</ISOAbbreviation></Journal><ArticleTitle>Use of new endophytic fungi as pretreatment to enhance enzymatic saccharification of Eucalyptus globulus.</ArticleTitle><Pagination><MedlinePgn>383-90</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.biortech.2015.07.088</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0960-8524(15)01059-7</ELocationID><Abstract><AbstractText>New endophytic fungi are assessed for the first time as pretreatment to enhance saccharification of Eucalyptus globulus wood. The fungi are all laccase-producing ascomycetes and were isolated from eucalyptus trees in Spain. After five endophytes had been assayed alone or in combination with white-rot fungus Trametes sp. I-62, three were pre-selected. To improve sugar production, an autohydrolysis pretreatment was performed before or after fungal treatment. Pretreatment increased sugar production 2.7 times compared to non-pretreated wood. When fungal and autohydrolysis pretreatments were combined, a synergistic increase in saccharification was observed in all cases. Endophytic fungi Ulocladium sp. and Hormonema sp. produced greater enhancements in saccharification than Trametes sp. I-62 (increase in sugar yields of 8.5, 8.0 and 6.0 times, respectively), demonstrating the high potential of these new endophytic fungi for saccharification enhancement.</AbstractText><CopyrightInformation>Copyright © 2015 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Martín-Sampedro</LastName><ForeName>Raquel</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>INIA - CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, Ctra. de La Coruña km 7.5, 28040 Madrid, Spain. Electronic address: martin.raquel@inia.es.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fillat</LastName><ForeName>Úrsula</ForeName><Initials>Ú</Initials><AffiliationInfo><Affiliation>INIA - CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, Ctra. de La Coruña km 7.5, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ibarra</LastName><ForeName>David</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>INIA - CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, Ctra. de La Coruña km 7.5, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Eugenio</LastName><ForeName>María E</ForeName><Initials>ME</Initials><AffiliationInfo><Affiliation>INIA - CIFOR, Forestry Products Department, Cellulose and Paper Laboratories, Ctra. de La Coruña km 7.5, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>07</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Bioresour Technol</MedlineTA><NlmUniqueID>9889523</NlmUniqueID><ISSNLinking>0960-8524</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber><NameOfSubstance UI="D042845">Laccase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001709" MajorTopicYN="N">Biotechnology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D050260" MajorTopicYN="Y">Carbohydrate Metabolism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060026" MajorTopicYN="N">Endophytes</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005052" MajorTopicYN="N">Eucalyptus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013030" MajorTopicYN="N">Spain</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055454" MajorTopicYN="N">Trametes</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Ascomycetes</Keyword><Keyword MajorTopicYN="N">Dothideomycetes</Keyword><Keyword MajorTopicYN="N">Endophytes</Keyword><Keyword MajorTopicYN="N">Enzymatic saccharification</Keyword><Keyword MajorTopicYN="N">Pretreatment</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>06</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>07</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>07</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>8</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>8</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26255602</ArticleId><ArticleId IdType="pii">S0960-8524(15)01059-7</ArticleId><ArticleId IdType="doi">10.1016/j.biortech.2015.07.088</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country><region><li>Communauté de Madrid</li></region></list><tree><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Martin Sampedro, Raquel" sort="Martin Sampedro, Raquel" uniqKey="Martin Sampedro R" first="Raquel" last="Martín-Sampedro">Raquel Martín-Sampedro</name></region><name sortKey="Eugenio, Maria E" sort="Eugenio, Maria E" uniqKey="Eugenio M" first="María E" last="Eugenio">María E. Eugenio</name><name sortKey="Fillat, Ursula" sort="Fillat, Ursula" uniqKey="Fillat U" first="Úrsula" last="Fillat">Úrsula Fillat</name><name sortKey="Ibarra, David" sort="Ibarra, David" uniqKey="Ibarra D" first="David" last="Ibarra">David Ibarra</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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